Developed specifically for nanospray mass spectrometry (MS), Eksigent NanoLC systems provide speed, sensitivity, resolution and reliability for proteomics research.
NanoLC systems employ Eksigent's Microfluidic Flow Control (MFC) technology, which creates rapid, reproducible, low-flow-rate binary gradients using continuous flow measurement in combination with a controlled pressure source. Measurement of temperature allows the NanoLC systems to adjust for viscosity changes over extended run times and produces excellent retention time reproducibility day-to-day and month-to-month, for improved identification of low-abundance proteins.
Since there is no flow splitting, flow inaccuracies and retention-time variability caused by splitter-based systems are eliminated. The figure below illustrates the excellent reproducibility that can be obtained using our nanoLC systems.
Six consecutive separations of a cytochrome C tryptic digest on a 75 µm ID nanoLC column at 200 nl/min. RSD on retention time is better than 0.4% for all peptides.
The system also incorporates Eksigent's peak parking capability, which allows the flow rate to be changed rapidly for higher speed sample loading or for extended MS/MS analysis to identify co-eluting and low abundance peptides. Binary solvent delivery, high pressure gradient mixing, autosampler, and PC-based instrument control are all available in our compact NanoLC systems.
With instruments designed for one-dimensional and two-dimensional applications, Eksigent NanoLC systems offer outstanding flexibility, throughput, and ease-of-use with Eksigent LC Control Software. And you can team up your NanoLC system with a mass spectrometer to improve ease-of-use, data integrity, and reliability. You can easily interface our NanoLC systems with the following mass spec software: Thermo Electron’s Xcalibur™, Applied Biosystems/MDS SCIEX Analyst®, and Bruker Daltonics’ HyStar™.
Check out our full line of NanoLC instrumentation.
Automated peak parking
Give your mass spectrometer more time to identify more proteins. With the NanoLC, you can perform peak parking without the need for additional valves or external pumps and increase identification of co-eluting or low-abundance peptides. Peak parking using NanoLC dynamic flow control significantly extends MS/MS acquisition time while maintaining the resolution of downstream peaks. Reducing the flow rate allows for extended secondary analysis, increasing the number of unique peptides that can be identified per run.
In addition to precise control of gradients at nanoscale flow rates, MFC allows you to change the flow rate dynamically for higher speed sample loading or for extended MS/MS analysis (peak parking) to identify co-eluting and low abundance peptides. After the gradient, the system can automatically increase flow rate to accelerate flushing and reduce the lag time between sample runs. Peak Parking can be simply triggered by a signal from your mass spectrometer, eliminating the complexity of additional pumps or valves.
For example, the mass spectrometer may see multiple co-eluting peptides that require more than the typical peak width to allow MS/MS on all of them. The mass spectrometer can then send a signal to the Eksigent NanoLC system and the NanoLC system can lower the gradient rapidly, or peak park, to increase the mass spectrometer acquisition time. Because the mass spectrometer in nano electrospray is concentration sensitive rather than mass sensitive, this change in flow rate does not degrade sensitivity. After the peptides have been analyzed, the NanoLC system can resume the gradients normal flow rate, without loss of resolution in the downstream peaks.
Eksigent’s MFC capability allows the flow rate to be changed rapidly for higher speed sample loading or for extended MS/MS analysis (peak parking) to identify co-eluting and low abundance peptides. After the gradient, DFC can automatically increase the flow rate to accelerate flushing and reduce the lag time between sample runs. Dynamic Flow Control can be triggered with one mouse click or by a signal from the mass spectrometer, eliminating the complexity of previous approaches requiring additional pumps or valves.
