ExpressLC® systems combine advances in gradient delivery, sample injection, and optical detection to deliver dramatically increased levels of chromatographic resolution and speed. Sample throughput can be increased by as much as 40% over conventional HPLC, making ExpressLC systems ideal for high throughput applications in drug discovery and development.
ExpressLC systems are known for their resolution, speed, and reliability. Eksigent’s direct-pumping Microfluidic Flow Control (MFC) technology provides rapid, precise adjustment of a variable-pressure pumping source. In-line flow meters actively measure the actual flow rate in each mobile phase and provide immediate adjustments to deliver an accurate, consistent and precise gradient, run-after-run.
With ExpressLC systems, you can expect accurate and precise gradients with every run and a level of speed and performance not achievable with conventional HPLC systems:
ExpressLC systems deliver dramatic increases in chromatographic resolution and speed via binary solvent delivery, high pressure gradient mixing, variable volume injection, UV absorbance detection, and PC-based instrument control in a compact, integrated package.
Get to MS Faster
ExpressLC systems with Microfluidic Flow Control (MFC) technology can help you add MS quickly by routing eluent from the UV detection cell through a short, direct transfer capillary to your ESI source. You can analyze the entire volume sample with no splitting or metering to cause instability or degrade resolution. ExpressLC systems are compatible with Thermo Electron’s Xcalibur™, Applied Biosystems/MDS SCIEX Analyst® and Bruker Daltonics’ HyStar™ mass spectrometer software.
Solve throughput problems
Available in single-channel and eight-channel formats, along with a reaction monitoring system, ExpressLC systems offer efficient, high-speed chromatography with excellent precision for gradient and isocratic separations. Small-scale HPLC columns, engineered specifically for the ExpressLC systems are also available.
Fully integrated control software
Eksigent’s Control Software makes the ExpressLC systems easy to learn, use, and maintain and gives you the flexibility to operate in manual or automated mode:
Microscale mixing
The ExpressLC incorporates a microscale mixer that has a volume of 300 nL, resulting in a mixing delay of only 2 seconds at 10 uL/min. This rapid mixing virtually eliminates the gradient delays inherent in conventional systems at the start of the method and at re-equilibrations. The rapid “deterministic” mixing leads to extremely precise gradients, rapid system response, and excellent gradient linearity.
Variable injection system
To scale down conventional methods to the ExpressLC system’s 300 micron i.d. columns, a typical 10 uL injection becomes 40 nL. There is no autosampler available that can deliver precise 40 nL injections, and our design philosophy required that the system provide conventional injection precision while using conventional autosamplers. To achieve this, we developed our own variable injection system. This system utilizes an electronically controlled injection valve coupled with our MFC flow delivery system. The autosampler merely needs to fill a sample loop (typically 250 nL) with sample. The valve is then toggled from load to inject and back to the load position, based on integrating the flow rate over time to reach the desired injection volume. This system provides excellent precision, variable volume injection while utilizing conventional autosampler technology.
Microscale UV flow cell
Scaling down a conventional UV absorbance detection system to capillary levels has typically resulted in increased dispersion and reduced detection sensitivity. Eksigent utilized its expertise in microfluidic chip development to develop a highly sensitive, non-dispersive UV absorbance flow cell. The detection system offers dynamic range and linearity, comparable to conventional detection systems. Furthermore, it offers array-based detection from 200 nm to 380 nm, providing full spectral information for each peak.
Learn more about Eksigent’s line of ExpressLC systems for pharmaceutical analysis and reaction monitoring:
Search a list of publication citations that describe how leading scientists are using Eksigent ExpressLC systems in drug discovery and development. Or learn more about the application of Eksigent’s analytical tools in drug discovery & development:
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