Dr. Allis Chien discusses how the use of extended length (60cm) capillary HPLC columns results in significant improvements in chromatographic resolution and peak shape, in comparison to using standard length columns.
Unfortunately, long columns are often regarded as a niche technique. The reality is that long column chromatography is extremely accessible, and is well suited to routine everyday use.
Practical information on implementing long column chromatography, including tips on column packing, are presented.
